Controls in Biosafety II Work Practices and Personal Protective Equipment

READING ASSIGNMENT:
BSP&P Ch. 19 & 20

OBJECTIVES:
By the end of the class, students will be able to:

• Describebest practices to follow when handling hazardous biological agents
• Definethe foundational work practice used to protect researchers handlinginfectious agents
• Outlinethe range of protective clothing and equipment options available forworker protection
• Discussrespiratory protection and review the range of respirators worn to protectresearchers against hazardous biological agents
• Reviewthe selection, use, limitations, reprocessing and disposal of protectiveclothing and equipment

POWERPOINT LECTURE TITLE:
Lecture 6

STUDENT HANDOUTS:
Work Practice documents and posters:

1. Biosafety Level 1 work practice poster, Biosafety Level 2 work practice poster
2. Biosafety Level 3 work practice poster, Toxin Safety Guidelines
3. Centrifuge Safety, Effective Use of the Biosafety Cabinet, Autoclave Safety, and
4. On-campus Transport.

The course host institution’s Institutional Biosafety Manual may serve as an alternate handout

IN-CLASS EXERCISES OR TOURS:

1. Biosafety Foundation Game
2. Student donning of protective clothing
3. Respirator Fit Testing practicum

Students would be given a description of the federally required elements of a respiratory protection program along with a hands-on opportunity to learn how to properly train, size, and qualitatively fit test a person for an air-purifying respirator.

Students would receive the opportunity to handle and wear air-purifying respirators, powered-air-purifying respirators and a self contained breathing apparatus (SCBA). This represents the full range of protective equipment that may be worn for protection against biohazards in both research and emergency response settings. Students would also have a chance to don additional emergency response protective clothing, such as booties, jump suits, gloves and Level B vapor tight protective suits.

FILMS:

Working Safely and Effectively in the Biological Safety Cabinet

CLASS DEMONSTRATION (EQUIPMENT/DEVICES):
Level B protective suit, Tyvek jump suit, various gloves, booties, N-95 respirator, N-100 respirator, powered-air-purifying-respirator, self-contained breathing apparatus, and respiratory protection fit testing kit

HOMEWORK:
None

ADDITIONAL REFERENCES:
None