Controls in Biosafety I: Facility Design and Containment Equipment

READING ASSIGNMENT:
BSP&P:  Ch. 14, 15, 16, & 17
BMBL:  App. A

OBJECTIVES:
By the end of the class, students will be able to:

• Explain the four Biosafety Levels for the containment of biological agents
• Describe the laboratory design features for each biocontainment level
• Define the concepts of primary and secondary containment
• Understand engineering controls utilized as primary containment devices
• Outline the types, features, operation, effective use, and limitations of biological safety cabinets

POWERPOINT LECTURE TITLE:
Lecture 5

STUDENT HANDOUTS:

None

IN-CLASS EXERCISES OR TOURS:
Containment Laboratory (Biosafety Level 3 Lab) Tour
To augment the BSL-3 Program course lecture, students may be toured through one of the campus (or local) containment laboratory locations with the course instructor along with facility management personnel.  A review of the layout of the facility, key physical safety features, lab entry, use, exit, and security controls should be provided for those on the tour.  Students may not be able to enter active BSL-3 research space, but would be able to view research laboratories from observation windows located in the anteroom and facility perimeter. 

FILMS:
Biosafety Level 3 for Service and Maintenance Personnel

CLASS DEMONSTRATION (EQUIPMENT/DEVICES):
None

HOMEWORK:
None

ADDITIONAL REFERENCES:

• NSF Standard #49:  Class II Biohazard Cabinetry
• CDC/NIH Primary Containment for Biohazards: Selection, installation and Use of Biological Safety Cabinets, 2000
• WHO Lab Biosafety Manual and Health Canada’s Laboratory Biosafety Guidelines (both available online under Biosafety Resources off the ABSA web page)