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Biosafety Level 3 Containment |
READING ASSIGNMENT:
None |
OBJECTIVES:
By the end of the class, students will be able to:
- Identify risks involved with the use of Risk Group 3 agents in research
- List training and experience required by personnel conducting research at Biosafety Level 3 containment
- Outline the range of personal protective equipment options for BSL3 research
- Demonstrate the required BSL3 work practices for both cell culture and animal experiments
- Describe the facility entry and exit requirements, including the donning and doffing of personal protective equipment required for BSL3 research
- Outline disinfection and decontamination procedures for the termination of BSL3 research
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POWERPOINT LECTURE TITLE:
Lecture 12 |
STUDENT HANDOUTS:
None |
IN-CLASS EXERCISES OR TOURS:
BSL-3 Facility Verification Tour/Exercise
Students may work with the course instructor and a representative from building services to test and verify the function of a BSL-3 containment laboratory. An overview of the verification process will be provided first along with a description of the monitoring equipment used to assess proper lab operation. Students will then gain direct experience with the airflow and pressure monitoring equipment to determine the exhaust and supply flow rates, airflow direction, strength of facility pressure differentials between rooms, and calculate the total number of air changes per hour of the facility. Upon completion of this exercise, students will have a firm understanding of the steps required for the annual verification and certification of BSL-3 containment laboratories |
FILMS:
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CLASS DEMONSTRATION (EQUIPMENT/DEVICES):
None |
HOMEWORK:
BSL3 Facility Verification Checklist (can be completed by the student group within class during the tour) |
ADDITIONAL REFERENCES:
None |
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